Student Project abstract The investigation of platinum-based drug effect on tumor stem cells responsible for tumor generation and propagation is a novel and expanding field, and in the colorectal cancer casuistry they are many features uncovered. Studies concerning the action of platinum compounds against immune mechanisms focusing activated lymphocytes involved could bring novelties in the field of cancer cell biochemistry and function.
Our aim is to perform a multidisciplinary and translational study that impact patient care in colorectal carcinoma. The project objective is to evaluate the platinum-containing drugs effect on stem cells in papilloma vescicale operazione carcinoma, this population being responsible for the resistance against chemotherapy treatment.
Colorectal cancer biomarkers is feasible to assess the platinum-treated stem cells, with accent on apoptosis, multidrug resistance, metabolism and cell signaling. The project intends to investigate the percent of stem cells having genetic mutations, and the effect of platinum-based compounds on cell populations which display chemoresistance versus targeted anti-EGFR therapy. We propose also to examine the lymphocyte populations implicated in antitumoral immune response, in order to establish the magnitude of immunomodulation following the platinum compounds action.
General objectives of the project A multidisciplinary assessment is proposed, which is capable to merge the scientific achievements of basic and clinical studies and to improve the results quality. Our aim is to perform a basic and translational study that impact patient care in colorectal carcinoma.
The project main objective is to evaluate the platinum-containing drugs impact on stem cells, these population being responsible for the tumoral dissemination and resistance against chemotherapy treatment. The clue of the treatment resides in the proportion of stem cells inhibited by the drug colorectal cancer biomarkers the whole tumoral population, and the project proposes to identify and characterize the changes which occur in colon carcinoma stem cells during platinum chemotherapy.
It is feasible to compare using functional genomic methods the platinum-treated colorectal cancer biomarkers and non-stem in the tumoral mass with accent on cells apoptosis, multidrug resistance, metabolism and cell signaling.
The project intends to investigate the percent of stem cells having colorectal cancer biomarkers mutations, and the effect of platinum-based compounds on EGFR-chemoresistant cell populations.
One of our objectives is to scan the lymphocyte populations implicated in antitumoral immune response, emphasizing the CD8 positive T lymphocytes and the natural killer cells, in order to establish the magnitude of the changes in human body immune response potential following the platinum compounds action. Lymphocytes from both peripheral blood and lymph nodes will be evaluated.
Microsatellite instability (MSI)
The proposed objectives were completed, bringing new, significant results in biochemistry of the platinum compounds, the most important metal involved in chemotherapy, but also the platinum-group metals PGMother metal complexes and their ligands showing therapeutic potential. We revealed multiple aspects of platinum-based chemotherapy, which join the outcomes of basic biochemical, genomic, proteomic and immune methods in order to improve the therapeutically colorectal cancer biomarkers.
After accomplishing all phases of the project, the final results of the project are: Scientific publications which acknowledges the project, indexed on Web of Science Thomson Reuters ISI: 1.
IF 0,; influency score 0, Synthesis of structural analogues of hexadecylphosphocholine and their antineoplastic, antimicrobial and amoebicidal activity. European Journal of Medicinal Chemistry, Imunomodulatory potential of palladium II complexes with 1E,6E -1,7-bis 3,4-dimethoxyphenyl hepta-1,6-diene-3,5-dione.
Notulae Botanicae Horti Agrobotanici,43 2 : colorectal cancer biomarkers Platinum derivatives: generic brands vs. Journal: Inorganic Chemistry Communications Elsevier, — Papers in journals indexed by international cancer de colon etapas Clujul Medical,88 3 : Ferdinand Devinsky from the Comenius University in Bratislava. Gregory S.
Cancerul la prostata
Virag, M. Perde-Schrepler, C.
Calciul din dieta zilnica si riscul de cancer Cancerul colorectal Legatura dintre cantitatea de calciu din dieta zilnica si riscul de cancer la colon nu a fost inca stabilita in mod definitiv, desi s-au facut mai multe studii in acest sens.
Tatomir, H. Decean, E.
Fischer-Fodor: UVB-activated signaling pathways and their modulation with Vitis vinifera seeds extract in human skin cells, Abstract Book, pages Virag, E. Improved oxygen-carrying blood substitutes in vitro effect on hematopoietic and endothelial cells.
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- Chiricuta" Cluj-Napoca, Romania Abstract Colorectal cancer is an important disease of the modern world, generating significant mortality and morbidity rates.
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Poster presentation. Virag, O. Balacescu, E.
Fischer-Fodor, M. Perde Schrepler, C. Tatomir, L.
Balacescu, I. Biochemical aspects of apoptosis and immunomodulation as a consequence of the treatment with platinum complexes and other metal-based compounds with antitumor therapeutic potential Through two main activities: 6. Evaluation of the molecules implicated in apoptotic signaling pathways in colorectal cells treated with metal complexes 6.
Assessment of the metal compounds effect on lymphocyte subsets and cellular colorectal cancer biomarkers molecules The final phase of the project, PHASE VI December 10th December 31st was implemented by completing the following objective: 6.
Evaluation of the molecules implicated in apoptotic signaling pathways in colorectal cells treated with metal complexes In order to identify new biomarkers connected to the apoptotic processes induced by metal compounds, the biologic effect of a new class of dysprosium complexes was studied; the antiproliferative effect of the Dy compounds was compared with that of standard platinum-based drugs.
The complex and its ligand toxicity were and we proved the decisive role of the central metal in the compounds biologic activity and in early apoptosis triggering.
The Genetics of Metastatic Colorectal Cancer
The treated tumor cell populations were exposed to a uniform magnetic filed, with an intensity which does not affected the cells viability, and we observed that unlike to the ligand or the platinum drugs, the magnetic field applied concomittant with in Dy III Na ampy 4]n treatment induced a stronger growth inhibitory effect on tumor populations. The cancer cells drug resistance was impeded by the simultaneous application colorectal cancer biomarkers the Dy complex and the magnetic field; this phenomenon was monitored through the evaluation of the MDR-1 biomarker of drug efflux in the cell, and a statistic significance was proved.